MALDI Imaging Mass Spectrometry
نویسندگان
چکیده
منابع مشابه
MALDI mass spectrometry imaging in oncology (Review).
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has improved over the years and is increasingly being used for biomarker discovery directly from human tissue sections. State-of-the-art technology currently enables a resolution down to 20 µm. MSI therefore allows the correlation of spatial and temporal protein expression profiles with distinct morphological fe...
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Recent developments in MALDI have enabled direct detection of lipids as intact molecular species present within cellular membranes. Abundant lipid-related ions are produced from the direct analysis of thin tissue slices when sequential spectra are acquired across a tissue surface that has been coated with a MALDI matrix. The lipid-derived ions can often be distinguished from other biomolecules ...
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BACKGROUND Three-dimensional (3D) imaging mass spectrometry (MS) is an analytical chemistry technique for the 3D molecular analysis of a tissue specimen, entire organ, or microbial colonies on an agar plate. 3D-imaging MS has unique advantages over existing 3D imaging techniques, offers novel perspectives for understanding the spatial organization of biological processes, and has growing potent...
متن کاملAtmospheric pressure molecular imaging by infrared MALDI mass spectrometry.
An atmospheric pressure (AP) MALDI imaging interface was developed for an orthogonal acceleration time-of-flight mass spectrometer and utilized to analyze peptides, carbohydrates, and other small biomolecules using infrared laser excitation. In molecular imaging experiments, the spatial distribution of mock peptide patterns was recovered with a detection limit of approximately 1 fmol/pixel from...
متن کاملMALDI mass spectrometry imaging of proteins exceeding 30,000 daltons.
BACKGROUND Since its introduction 10 years ago by Caprioli and associates, MALDI mass spectrometry imaging has enabled spatial analysis of drugs, lipids, peptides, and polypeptides. In polypeptides, the detectable mass range is limited to small proteins with a mass less than 25 kDa. This is a limitation, as many proteins, including cytokines, growth factors, enzymes, and receptors have molecula...
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2009
ISSN: 1535-9476
DOI: 10.1074/mcp.r800016-mcp200